Product Name: ORO-ZONE

Type: Oral Water-Soluble Powder - Antidiarrheal Antibiotic Combination

COMPOSITION

Each 12 g contains:

• Neomycin Sulphate BP ...... 400 mg
• Streptomycin Sulphate BP ...... 400 mg
• Sulphaguanidine BP ...... 4 g
• Kaolin BP ...... 4 g
• Pectin USP ...... 400 mg
• Bismuth Subnitrate USP ...... 2 g
• Vitamin A Acetate BP ...... 80,000 IU

DESCRIPTION

ORO-ZONE is a comprehensive, broad-spectrum antidiarrheal powder formulated for the treatment and control of bacterial enteritis and gastrointestinal infections in poultry. This multi-action formulation combines systemic and locally acting antibacterials with adsorbents, protective agents, and vitamin A to address both the infectious causes of diarrhea and its clinical consequences .

Neomycin and streptomycin are aminoglycoside antibiotics that act synergistically against gram-negative enteric pathogens by binding to the 30S ribosomal subunit, inhibiting bacterial protein synthesis and causing cell death . Sulphaguanidine is a poorly absorbed sulfonamide that acts locally in the intestinal tract, providing effective antibacterial activity against enteric bacteria .

Kaolin, pectin, and bismuth subnitrate work together as adsorbents and protective agents, binding bacterial toxins, reducing fluid loss, and providing a soothing coating to inflamed intestinal mucosa . Vitamin A acetate supports epithelial integrity and immune function during recovery from enteric infections .

MECHANISM OF ACTION

Neomycin Sulphate:

• Binds to the 30S ribosomal subunit, inhibiting bacterial protein synthesis
• Causes misreading of mRNA, leading to production of non-functional proteins and cell death
• Poorly absorbed from the gastrointestinal tract, exerting its primary effect in the gut lumen

Streptomycin Sulphate:

• Also binds to the 30S ribosomal subunit, blocking bacterial protein synthesis
• Acts synergistically with neomycin against gram-negative enteric pathogens
• Provides broader spectrum coverage when combined with neomycin

Sulphaguanidine:

• Competitively inhibits bacterial folate synthesis by blocking para-aminobenzoic acid (PABA)
• Poorly absorbed from the gastrointestinal tract, providing high local concentrations in the gut
• Effective against a wide range of enteric bacteria, including E. coli and Salmonella spp.

Kaolin:

• Acts as an adsorbent, binding to bacterial toxins, endotoxins, and irritants in the gastrointestinal tract
• Provides a protective coating to inflamed intestinal mucosa
• Helps reduce fluid loss and firm stools

Pectin:

• Soluble fiber that absorbs water and helps firm stools
• Provides a soothing, protective coating to the intestinal lining
• Supports normal bowel function

Bismuth Subnitrate:

• Provides a protective coating to inflamed intestinal mucosa
• Has mild astringent properties that help reduce inflammation
• May have mild antibacterial activity against enteric pathogens

Vitamin A Acetate:

• Essential for maintaining the integrity of intestinal epithelial cells
• Supports immune function during recovery from infection
• Helps repair damaged intestinal mucosa

ANTIMICROBIAL SPECTRUM

ORO-ZONE is effective against a broad range of enteric pathogens:

Gram-Negative Bacteria:

• Escherichia coli - colibacillosis, enteritis
• Salmonella spp. - salmonellosis, paratyphoid infections
• Shigella spp.
• Klebsiella spp.
• Proteus spp.

Gram-Positive Bacteria:

• Staphylococcus spp. (variable sensitivity)
• Streptococcus spp.

INDICATIONS

ORO-ZONE is indicated for the treatment and control of gastrointestinal infections and diarrhea in poultry caused by neomycin, streptomycin, and sulphaguanidine-sensitive bacteria .

Disease Conditions:

• Bacterial Enteritis - inflammation of the intestines caused by bacterial pathogens
• Colibacillosis - E. coli infections affecting the gastrointestinal tract
• Salmonellosis - including paratyphoid infections in poultry
• Diarrhea and Scours - acute and chronic diarrhea of bacterial origin
• Secondary bacterial enteritis associated with viral or parasitic infections
• Nonspecific bacterial diarrhea in growing birds

DOSAGE & ADMINISTRATION

ORO-ZONE is administered via drinking water or mixed with feed. For optimal results, fresh medicated water should be prepared daily. Shake well before use.

Recommended Dosage:

• Poultry (Broilers, Layers, Breeders): 12 g per 10-15 liters of drinking water (approximately 1 g per 1-1.5 liters) for 3-5 consecutive days
• Alternative Dosage (feed mixing): 12 g per 5-10 kg of feed, administered as the sole ration for 3-5 days
• Severe Infections: Double the dosage for the first 24 hours, then continue with standard dosage
• Treatment Duration: 3-5 consecutive days. For chronic conditions, extend up to 7 days if necessary

Administration Guidelines:

• Calculate total daily water consumption based on flock size, age, and environmental temperature
• Dissolve the required amount of ORO-ZONE in a small volume of water, then add to the main drinking water tank
• For use in automatic water proportioners, prepare stock solution according to manufacturer specifications
• Prepare fresh medicated water daily; discard any unused solution after 24 hours
• Ensure medicated water is the sole source of drinking water during treatment
• For best results, administer over 4-8 hours; may withhold water for 1-2 hours before treatment to encourage rapid consumption
• If symptoms persist beyond 3-4 days, consult a veterinarian

EFFICACY DATA

Combination Therapy Studies:

• A study examining antimicrobial therapies in chicks infected with Salmonella infantis found that neomycin-based combination therapies were effective in controlling infection .
• The combination of neomycin with other antimicrobials helped reduce the number of infected chicks compared to control groups .

Role of Adsorbents:

• Kaolin and pectin are well-established adsorbents that bind bacterial toxins and provide a protective coating to inflamed intestinal mucosa
• These components are particularly useful as supportive therapy in infectious diarrhea, helping to reduce fluid loss and promote recovery

DRUG INTERACTIONS

• Kaolin and pectin: Do not administer kaolin and pectin with lincomycin as they may inhibit the oral absorption of lincomycin
• Aminoglycosides: Concurrent use with other aminoglycosides (gentamicin, tobramycin) may increase the risk of nephrotoxicity and ototoxicity
• Sulphonamides: May potentiate the effects of anticoagulants; monitor if used concurrently
• Vitamin A: No known significant interactions; compatible with most other feed additives

CONTRAINDICATIONS & PRECAUTIONS

• Egg production: Do not use in laying hens producing eggs for human consumption. Neomycin, streptomycin, and sulphaguanidine can accumulate in eggs .
• Hypersensitivity: Do not use in animals with known hypersensitivity to aminoglycosides (neomycin, streptomycin), sulfonamides, or any component
• Renal impairment: Use with caution in animals with impaired renal function; aminoglycosides are nephrotoxic
• Ruminants: Use with caution in ruminating animals; kaolin and pectin may interfere with rumen fermentation
• Withdrawal period: Observe the recommended withdrawal period to ensure consumer safety
• Keep out of reach of children
• Store in a cool, dry place below 30°C, protected from direct sunlight
• Protect from moisture
• Use within 28 days after first opening

ADVERSE REACTIONS

• At recommended doses, the combination is generally well tolerated in poultry
• Rarely, aminoglycosides may cause ototoxicity (hearing loss) and nephrotoxicity (kidney damage) with systemic absorption
• Sulfonamides may cause crystalluria; ensure adequate water intake during treatment
• If adverse reactions occur, discontinue use and consult a veterinarian

WITHDRAWAL PERIOD

• Meat (Poultry): 7-10 days (consult local regulatory requirements)
• Eggs: Not for use in laying hens producing eggs for human consumption
• Note: Withdrawal periods may vary by country; always follow local regulatory requirements

STORAGE

• Store in a cool, dry place below 30°C
• Protect from direct sunlight and moisture
• Keep container tightly closed when not in use
• Do not freeze

Target Species: Poultry (Broilers, Layers, Breeders, Turkeys, Ducks, Geese, Quail)

Pack Sizes: 1 Kg HDPE container

PRODUCT SUMMARY
ORO-ZONE Powder Antibiotic Neomycin Sulphate 400mg, Streptomycin Sulphate 400mg, Sulphaguanidine 4g, Kaolin 4g, Pectin 400mg, Bismuth Subnitrate 2g, Vitamin A Acetate 80,000 IU per 12g Poultry 1 Kg

FOR VETERINARY USE ONLY