Product Name: TRI-BIT

Type: Oral Water-Soluble Powder - Antibiotic Combination

COMPOSITION

Each 100 g contains:

• Neomycin (as Neomycin Sulphate BP) ...... 7 g (7% w/w, 70 mg per gram)
• Chlortetracycline Hydrochloride USP ...... 8 g (8% w/w, 80 mg per gram)
• Colistin Sulfate BP ...... 0.4 g (0.4% w/w, 4 mg per gram)

DESCRIPTION

TRI-BIT is a broad-spectrum antibiotic combination powder containing neomycin, chlortetracycline, and colistin. This triple-action formulation provides enhanced antibacterial activity against both gram-positive and gram-negative pathogens, making it an effective treatment for enteric and respiratory infections in poultry .

Neomycin is an aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, causing misreading of mRNA and production of non-functional proteins . Chlortetracycline is a tetracycline antibiotic that also binds to the 30S ribosomal subunit, blocking the attachment of aminoacyl-tRNA to the mRNA-ribosome complex . Colistin is a polymyxin antibiotic that disrupts the bacterial cell membrane, causing leakage of cellular contents and cell death .

This combination provides synergistic coverage against a wide range of pathogens, including Escherichia coli, Salmonella spp., Pasteurella multocida, and other susceptible bacteria

MECHANISM OF ACTION

Neomycin:

• Binds to the 30S ribosomal subunit, inhibiting bacterial protein synthesis
• Causes misreading of mRNA, leading to production of non-functional proteins and cell death
• Poorly absorbed from the gastrointestinal tract, exerting its primary effect in the gut lumen
• Effective against a wide range of gram-negative bacteria

Chlortetracycline:

• Binds reversibly to the 30S ribosomal subunit, preventing the attachment of aminoacyl-tRNA to the mRNA-ribosome complex
• Inhibits bacterial protein synthesis with broad-spectrum activity
• Achieves high concentrations in respiratory and intestinal tissues
• Active against both gram-positive and gram-negative bacteria, as well as Mycoplasma

Colistin:

• Acts as a cationic surface-active agent, binding to lipopolysaccharides in the outer membrane of gram-negative bacteria
• Displaces divalent cations (Ca²⁺, Mg²⁺), destabilizing the cell membrane
• Increases membrane permeability, leading to leakage of cellular contents and cell death
• Poorly absorbed from the gastrointestinal tract, exerting its primary effect in the gut lumen

Synergistic Combination:

• The combination of neomycin and chlortetracycline provides enhanced activity against both gram-positive and gram-negative bacteria through complementary inhibition of the 30S ribosomal subunit
• Colistin extends the spectrum to include colistin-susceptible gram-negative bacteria and enhances membrane permeability, potentially improving the efficacy of the other antibiotics
• This triple-action formulation provides comprehensive coverage for both respiratory and gastrointestinal infections

ANTIMICROBIAL SPECTRUM

TRI-BIT is effective against a broad range of microorganisms:

Gram-Negative Bacteria:

• Escherichia coli - colibacillosis, enteritis
• Salmonella spp. - salmonellosis, fowl typhoid, pullorum disease
• Pasteurella multocida - fowl cholera
• Haemophilus paragallinarum - infectious coryza
• Klebsiella spp., Proteus spp., Shigella spp.

Gram-Positive Bacteria:

• Staphylococcus spp.
• Streptococcus spp.
• Clostridium spp.

Mycoplasma:

• Mycoplasma gallisepticum - chronic respiratory disease (CRD)
• Mycoplasma synoviae - infectious synovitis

INDICATIONS

TRI-BIT is indicated for the treatment and control of enteric and respiratory infections in poultry caused by neomycin, chlortetracycline, and colistin-sensitive bacteria

Disease Conditions:

• Colibacillosis - E. coli infections causing enteritis, airsacculitis, pericarditis, and septicemia
• Salmonellosis - fowl typhoid, pullorum disease, paratyphoid infections
• Chronic Respiratory Disease (CRD) - mycoplasmosis with secondary bacterial infections
• Fowl Cholera - Pasteurella multocida infection
• Infectious Coryza - Haemophilus paragallinarum infection
• Bacterial Enteritis - diarrhea of bacterial origin
• Mixed bacterial infections involving both gram-positive and gram-negative pathogens
• Secondary bacterial infections associated with viral diseases

DOSAGE & ADMINISTRATION

TRI-BIT is administered via drinking water. For optimal results, fresh medicated water should be prepared daily.

Recommended Dosage:

• Poultry (Broilers, Layers, Breeders): 1 g per 2-4 liters of drinking water for 3-5 consecutive days
• Alternative Dosage (body weight): 20-30 mg neomycin + 30-40 mg chlortetracycline + 2-3 mg colistin per kg body weight per day
• Treatment Duration: 3-5 consecutive days. For chronic infections, up to 7 days may be required

Administration Guidelines:

• Calculate total daily water consumption based on flock size, age, and environmental temperature
• Dissolve the required amount of TRI-BIT in a small volume of water, then add to the main drinking water tank
• For use in automatic water proportioners, prepare stock solution according to manufacturer specifications
• Prepare fresh medicated water daily; discard any unused solution after 24 hours
• Ensure medicated water is the sole source of drinking water during treatment
• For best results, administer over 4-8 hours; may withhold water for 1-2 hours before treatment to encourage rapid consumption
• Do not use with bacteriostatic antibiotics that may cause antagonism
• Avoid concurrent use with calcium, magnesium, iron, or aluminum-containing products as they may reduce chlortetracycline absorption

EFFICACY AND SYNERGY DATA

Synergistic Activity:

• The combination of neomycin and chlortetracycline provides enhanced activity against both gram-positive and gram-negative bacteria through complementary inhibition of the 30S ribosomal subunit
• Colistin enhances the efficacy by disrupting bacterial cell membranes, potentially allowing better penetration of the other antibiotics

Residue Study:

• A 2025 study on antibiotic residue elimination in Taihang chickens established that the recommended withdrawal time for chlortetracycline is 5 days . For neomycin, withdrawal periods of 7-14 days are typically recommended depending on regulatory jurisdiction .

CONTRAINDICATIONS & PRECAUTIONS

• Egg production: Do not use in laying hens producing eggs for human consumption. Neomycin and chlortetracycline can accumulate in eggs .
• Hypersensitivity: Do not use in animals with known hypersensitivity to aminoglycosides (neomycin), tetracyclines (chlortetracycline), or polymyxins (colistin)
• Renal impairment: Use with caution in animals with impaired renal function; aminoglycosides are nephrotoxic
• Young birds: Tetracyclines may cause discoloration of teeth in young animals; use with caution in very young chicks
• Drug interactions: Do not use concurrently with other nephrotoxic drugs. Avoid concurrent use with calcium, magnesium, iron, or aluminum-containing products as they may reduce chlortetracycline absorption
• Keep out of reach of children
• When handling the product, avoid inhalation, oral exposure, and direct contact with skin or eyes
• Store in a cool, dry place below 30°C, protected from direct sunlight
• Protect from moisture
• Use within 28 days after first opening

ADVERSE REACTIONS

• At recommended doses, the combination is generally well tolerated in poultry
• Occasional mild diarrhea may occur due to intestinal flora changes
• Prolonged use of neomycin may cause malabsorption syndrome in the gut
• Discoloration of teeth in young animals with prolonged tetracycline use
• If adverse reactions occur, discontinue use and consult a veterinarian

WITHDRAWAL PERIOD

• Meat (Poultry): 14 days (based on the longer withdrawal requirements for neomycin)
• Eggs: Not for use in laying hens producing eggs for human consumption
• Note: Withdrawal periods may vary by country; always follow local regulatory requirements. Neomycin typically requires 7-14 days, chlortetracycline requires 5 days

STORAGE

• Store in a cool, dry place below 30°C
• Protect from direct sunlight and moisture
• Keep container tightly closed when not in use
• Do not freeze

Target Species: Poultry (Broilers, Layers, Breeders, Turkeys, Ducks, Geese, Quail)

Pack Sizes: 1 Kg HDPE container

PRODUCT SUMMARY
TRI-BIT Powder Antibiotic Neomycin as Sulphate BP 7g, Chlortetracycline HCl USP 8g, Colistin Sulphate BP 0.4g per 100g Poultry 1 Kg

FOR VETERINARY USE ONLY