Product Name: DOX-T-ZONE

Type: Oral Water-Soluble Powder - Antibiotic Combination

COMPOSITION

Each 100 g contains:

• Doxycycline Hyclate ...... 210 g (21% w/w, 2100 mg per gram)
• Tylosin Tartrate ...... 100 g (10% w/w, 1000 mg per gram)

Note: This product contains an error in the provided composition. Based on industry standards, typical doxycycline:tylosin combination products contain 10-20% doxycycline and 10% tylosin [citation:2][citation:3]. The composition above reflects the exact percentages as provided, which may represent a concentrated formulation or require confirmation with the manufacturer.

DESCRIPTION

DOX-T-ZONE is a high-concentration, broad-spectrum antibiotic combination powder containing doxycycline hyclate and tylosin tartrate. This synergistic formulation provides enhanced antibacterial activity against both gram-positive and gram-negative bacteria, as well as Mycoplasma species, making it a cornerstone treatment for respiratory diseases in poultry [citation:1][citation:2].

Doxycycline is a semi-synthetic tetracycline with broad-spectrum bacteriostatic activity against a wide range of gram-positive and gram-negative bacteria, as well as Mycoplasma, Chlamydia, and Rickettsia species. Tylosin is a macrolide antibiotic derived from Streptomyces fradiae with potent activity against Mycoplasma species and gram-positive bacteria. The combination of these two antibiotics displays a synergistic effect against susceptible microorganisms, making it particularly effective for treating complex respiratory infections [citation:1][citation:2].

MECHANISM OF ACTION

Doxycycline:

• Inhibits bacterial protein synthesis by binding reversibly to the 30S ribosomal subunit [citation:2]
• Prevents the attachment of aminoacyl-tRNA to the mRNA-ribosome complex, blocking the addition of new amino acids to the growing peptide chain
• Exhibits bacteriostatic activity with broad-spectrum coverage against gram-positive and gram-negative bacteria [citation:1]
• Well absorbed after oral administration; widely distributed throughout body tissues

Tylosin:

• Inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit [citation:2]
• Interferes with peptidyl transferase activity, preventing peptide bond formation
• Exhibits primarily bacteriostatic activity with bactericidal effects at higher concentrations [citation:6]
• Particularly effective against Mycoplasma species and gram-positive bacteria [citation:1]

Synergistic Combination:

• The combination of doxycycline and tylosin acts synergistically, providing enhanced antimicrobial activity due to their complementary action on the ribosome (30S and 50S subunits) [citation:1][citation:2]
• This dual inhibition of bacterial protein synthesis provides broader coverage and helps reduce antibiotic resistance development [citation:1]
• The combination is particularly effective against mixed infections involving Mycoplasma and enteric bacteria [citation:3]

ANTIMICROBIAL SPECTRUM

DOX-T-ZONE is effective against a broad range of microorganisms:

Mycoplasma:

• Mycoplasma gallisepticum - chronic respiratory disease (CRD) [citation:3][citation:10]
• Mycoplasma synoviae - infectious synovitis, respiratory disease [citation:3]
• Mycoplasma meleagridis - in turkeys

Gram-Positive Bacteria:

• Staphylococcus spp. [citation:2][citation:6]
• Streptococcus spp. [citation:2]
• Clostridium perfringens [citation:2]
• Bacillus anthracis, Corynebacterium spp. [citation:2]

Gram-Negative Bacteria:

• Escherichia coli - colibacillosis [citation:2][citation:3]
• Salmonella spp. - salmonellosis [citation:2]
• Pasteurella multocida - fowl cholera [citation:2][citation:3]
• Haemophilus paragallinarum - infectious coryza [citation:3]
• Bordetella spp., Campylobacter spp., Klebsiella spp. [citation:2]

INDICATIONS

DOX-T-ZONE is indicated for the treatment and control of respiratory and gastrointestinal infections in poultry caused by doxycycline and tylosin-sensitive bacteria [citation:1][citation:2].

Disease Conditions:

• Chronic Respiratory Disease (CRD) - mycoplasmosis caused by Mycoplasma gallisepticum [citation:3][citation:10]
• Complex Chronic Respiratory Disease (CCRD) - mixed infections involving Mycoplasma and E. coli [citation:1][citation:3]
• Infectious Coryza - Haemophilus paragallinarum infection [citation:3]
• Fowl Cholera - Pasteurella multocida infection [citation:2][citation:3]
• Colibacillosis - E. coli infections affecting the respiratory and gastrointestinal tracts [citation:2][citation:3]
• Salmonellosis - fowl typhoid, pullorum disease, paratyphoid infections [citation:2]
• Ornithobacterium rhinotracheale (ORT) infection [citation:3]
• Sinusitis and Synovitis - in turkeys and chickens [citation:3]
• Secondary bacterial infections associated with viral diseases [citation:1]

DOSAGE & ADMINISTRATION

DOX-T-ZONE is administered via drinking water. For optimal results, fresh medicated water should be prepared daily.

Recommended Dosage:

• Poultry (Broilers, Layers, Breeders, Turkeys): 0.5 - 1 g per 2-4 liters of drinking water for 3-5 consecutive days [citation:2][citation:3][citation:7]
• Alternative Dosage (body weight): 1 g per 10-20 kg body weight per day [citation:2][citation:3]
• Treatment Duration: 3-5 consecutive days. For chronic or severe infections, up to 7 days may be required [citation:1][citation:2]

Dosage Calculation:

• At 1 g per 2-4 liters of water: Each kg of product medicates 2000-4000 liters of drinking water
• With the high concentration (2100 mg doxycycline + 1000 mg tylosin per gram), careful dosing is recommended

Administration Guidelines:

• Calculate total daily water consumption based on flock size, age, and environmental temperature
• Dissolve the required amount of DOX-T-ZONE in a small volume of water, then add to the main drinking water tank
• For use in automatic water proportioners, prepare stock solution according to manufacturer specifications
• Prepare fresh medicated water daily; discard any unused solution after 24 hours [citation:2]
• Ensure medicated water is the sole source of drinking water during treatment [citation:2]
• For best results, administer over 4-8 hours; may withhold water for 1-2 hours before treatment to encourage rapid consumption
• Do not use water containing high levels of calcium, magnesium, or iron as these may reduce absorption [citation:2]

RESIDUE AND WITHDRAWAL STUDIES

Scientific Residue Study:

• A peer-reviewed study published in the Journal of Food Science (2021) evaluated residues of doxycycline and tylosin mixture in broiler chickens. Both antibiotics were found to be more persistent in liver tissues than in muscle and fat. The study emphasized the importance of adhering to proper withdrawal periods to ensure consumer safety [citation:4][citation:8].
• Residues in fat persisted for a longer time than in muscle for tylosin, while the reverse was observed for doxycycline [citation:4].
• Cooking led to a marked decline in residue levels, while freezing had little effect [citation:4].

DRUG INTERACTIONS

• Antagonistic: Do not use concurrently with bactericidal antibiotics such as penicillins, cephalosporins, quinolones, or cycloserine [citation:1][citation:2][citation:6].
• Absorption interference: Tetracyclines chelate divalent or trivalent cations such as calcium, iron, magnesium, and aluminum. Concurrent administration of antacids, kaolin, pectin, or feed containing high levels of these minerals may decrease gastrointestinal absorption [citation:2].
• Synergistic: The combination of doxycycline and tylosin itself is synergistic; no other significant synergistic interactions documented [citation:1].

CONTRAINDICATIONS & PRECAUTIONS

• Egg production: Do not use in laying hens producing eggs for human consumption. Use is forbidden in laying hens [citation:2][citation:3].
• Hypersensitivity: Do not use in animals with known hypersensitivity to tetracyclines, macrolides, or any component [citation:1][citation:2].
• Hepatic and renal impairment: Do not use in animals with severe liver or kidney dysfunction [citation:1][citation:2].
• Young animals: Use with caution in very young animals; tetracyclines may cause discoloration of teeth [citation:1][citation:2].
• Pregnancy and lactation: Not recommended for use during pregnancy and lactation [citation:2].
• Sensitive species: Do not allow horses, rabbits, hamsters, guinea pigs, or ruminants access to water containing this product [citation:1].
• Keep out of reach of children
• When handling the product, avoid inhalation, oral exposure, and direct contact with skin or eyes. People with known hypersensitivity to tetracyclines or macrolides should avoid contact.
• Store in a cool, dry place below 30°C, protected from direct sunlight and moisture [citation:1][citation:3].
• Use within 28 days after first opening; medicated water should be used within 24 hours [citation:2].

ADVERSE REACTIONS

• At recommended doses, the combination is generally well tolerated in poultry [citation:2].
• Occasional mild diarrhea may occur due to intestinal flora changes [citation:1].
• Allergic reactions such as dermatitis, itching, erythema, or respiratory phenomena may occur in rare cases [citation:2].
• Discoloration of teeth in young animals with prolonged use [citation:1].
• If adverse reactions occur, discontinue use and consult a veterinarian.

WITHDRAWAL PERIOD

• Meat (Poultry): 7-15 days (varies by regulatory jurisdiction). Standard: 7-8 days [citation:3][citation:4][citation:7]. Some sources recommend 15 days [citation:1].
• Eggs: 4 days [citation:1][citation:3].
• Note: Withdrawal periods may vary by country; always follow local regulatory requirements. The 2021 residue study confirmed that residues persist in liver tissue longer than in muscle, emphasizing the importance of proper withdrawal periods [citation:4][citation:8].

STORAGE

• Store in a cool, dry place below 30°C [citation:1][citation:3].
• Protect from direct sunlight and moisture [citation:1].
• Keep container tightly closed when not in use [citation:2].
• Do not freeze.

Target Species: Poultry (Broilers, Layers, Breeders, Turkeys, Ducks, Geese, Quail) - Not for use in laying hens producing eggs for human consumption [citation:2][citation:3]

Pack Sizes: 1 Kg HDPE container

PRODUCT SUMMARY
DOX-T-ZONE Powder Antibiotic Doxycycline Hyclate 210g, Tylosin tartrate 100g per 100g Poultry 1 Kg

FOR VETERINARY USE ONLY