Product Name: SUPER HYDROXY D

Type: Oral Liquid Solution - Nutraceutical (Vitamin D3 Metabolite)

COMPOSITION

Each ml contains:

• 25-Hydroxycholecalciferol (25-OH-D3) ...... 7.4 µg (micrograms)

DESCRIPTION

SUPER HYDROXY D is a concentrated liquid supplement containing 25-hydroxycholecalciferol (25-OH-D3), the primary circulating and active metabolite of vitamin D3. Unlike conventional vitamin D3, which requires hydroxylation in the liver to become biologically active, 25-OH-D3 bypasses this conversion step, providing enhanced bioavailability and more rapid tissue uptake [citation:4].

25-Hydroxycholecalciferol is the major circulating form of vitamin D and is used as a more bioavailable alternative to traditional vitamin D3 supplementation in poultry [citation:4]. Studies have demonstrated that 25-OH-D3 is approximately 1.6–1.9 times more effective than vitamin D3 for improving laying performance and eggshell quality [citation:4].

MECHANISM OF ACTION

Superior Bioavailability:

• 25-OH-D3 bypasses hepatic 25-hydroxylase conversion, making it more readily available for absorption and utilization [citation:4]
• Has greater polarity than vitamin D3, resulting in enhanced intestinal absorption [citation:9]
• Exhibits higher affinity for vitamin D-binding proteins, leading to more efficient transport in the bloodstream [citation:9]

Calcium and Phosphorus Metabolism:

• Regulates calcium and phosphorus absorption in the intestine
• Enhances calcium mobilization from bone stores
• Supports renal reabsorption of calcium and phosphorus
• 25-OH-D3 supplementation significantly increases serum 25-OH-D3 levels, improves growth performance, and optimizes Ca and P utilization in broilers fed deficient diets [citation:9]

Intestinal and Bone Health:

• 25-OH-D3 supplementation improves jejunal morphology, increasing villus height and villus-to-crypt ratio [citation:10]
• Supports tight junction protein expression (ZO-1, Claudin-1), enhancing intestinal barrier function [citation:1]
• Improves tibia quality, including fresh tibia weight, breaking strength, and mineral content [citation:10]
• Increases bone ash, calcium, and phosphorus content [citation:9]

INDICATIONS & BENEFITS

SUPER HYDROXY D is indicated for the support of calcium metabolism, bone health, and eggshell quality in poultry

Broiler Chickens:

• Enhanced Growth Performance: Supplementation of 25-OH-D3 (69 µg/kg) significantly increases body weight gain (BWG) and reduces feed conversion ratio (FCR) in broilers from 1 to 21 days of age [citation:9]
• Improved Bone Health: Increases tibia breaking strength, bone ash content, and mineralization, reducing the incidence of leg problems [citation:9][citation:10]
• Optimal Performance: The relative bioavailability of 25-OH-D3 compared to vitamin D3 is 138-195% based on growth performance parameters [citation:4]

Layer Chickens:

• Superior Eggshell Quality: 25-OH-D3 supplementation significantly increases eggshell strength, eggshell thickness, and relative eggshell weight [citation:4][citation:8]
• Enhanced Eggshell Ultrastructure: Increases palisade layer thickness and mammillary knob density, improving shell integrity [citation:8]
• Improved Laying Performance: 25-OH-D3 (125 µg/kg) increases laying rate and reduces unqualified egg rate and mortality in late period laying hens [citation:8]
• Egg Vitamin D Enrichment: Co-supplementation of vitamin D3 with 25-OH-D3 significantly increases egg vitamin D content, with levels reaching 8.52 µg per whole egg—nearly 39-fold greater than unsupplemented controls [citation:1]
• Relative Bioavailability: 25-OH-D3 is approximately 1.6–1.9 times as effective as vitamin D3 for laying performance and eggshell quality [citation:4]

Breeder Chickens:

• Improved Fertility and Hatchability: 25-OH-D3 supplementation (69 mg/ton) reduces embryo mortality and improves hatchability in broiler breeder hens [citation:3]
• Enhanced Eggshell Quality: Improves eggshell specific gravity, indicating better shell quality [citation:3]

General Health Benefits:

• Immune Support: 25-OH-D3 supplementation modulates systemic inflammation, reducing C-reactive protein (CRP) levels and improving vitamin D status [citation:1]
• Intestinal Health: Enhances tight junction gene expression (ZO-1, Claudin-1) and improves villus morphology, strengthening intestinal barrier function [citation:1][citation:10]
• Antioxidant Status: Improves antioxidant enzyme activities and free radical scavenging abilities in aged laying hens [citation:10]
• Stress Reduction: Decreases cortisol levels and improves overall physiological status during periods of stress [citation:10]

SCIENTIFIC EVIDENCE

Relative Bioavailability Study (2025):

• A 2025 study in Poultry Science evaluated the relative bioavailability of 25-OH-D3 compared to vitamin D3 in laying hens from 41 to 48 weeks of age. Results showed that 25-OH-D3 was approximately 1.6–1.9 times as effective as vitamin D3 for laying performance and eggshell quality. Taking vitamin D3 as 100%, 25-OH-D3 had relative biological values of 135.9–195.9% for laying performance and 93–323.5% for eggshell quality [citation:4].

Egg Enrichment and Gut Health Study (2025):

• A 2025 study in Poultry Science evaluated co-supplementation of vitamin D3 with 25-OH-D3 in late-stage laying hens. Co-supplementation significantly improved hen-day egg production, yolk proportion, and Haugh unit. Vitamin D enrichment reached 8.52 µg per whole egg—nearly 39-fold greater than unsupplemented controls. Serum 25(OH)D concentrations increased while C-reactive protein decreased, indicating reduced systemic inflammation. Intestinal morphology and tight junction gene expression (ZO-1, Claudin-1) were also enhanced [citation:1].

Late-Phase Layer Study (2024):

• A 2024 study in Animals demonstrated that dietary 25-OHD3 supplementation (50 μg/kg) with 2000 IU/kg vitamin D3 significantly increased laying rate, eggshell strength, albumen height, and Haugh unit. The supplementation improved jejunal morphology (increased villus height to crypt depth ratio), tibia quality (fresh weight, strength, mineral content), and antioxidant status in aged laying hens [citation:10].

Broiler Performance Study (2024):

• A 2024 study in animal showed that 25-OH-D3 supplementation (69 μg/kg) increased body weight gain and reduced feed conversion ratio from 1 to 21 days in broilers. Serum 25-OH-D3 levels were significantly increased, and improvements in growth performance were observed despite Ca- and P-deficient diets [citation:9].

Comparison Study (2022):

• A 2022 study in Animals compared high doses of 25-OH-D3 (125 µg/kg) with high doses of vitamin D3 (125 µg/kg) in late period laying hens. Results showed that high supplementary doses of either VD3 or 25-OHD3 increased laying rate, decreased unqualified egg rate, and improved eggshell strength, eggshell thickness, palisade layer thickness, and plasma calcium levels. There was an equal effect on laying performance and eggshell quality in hens fed dietary 125 µg/kg doses of VD3 or 25-OHD3 [citation:8].

REGULATORY STATUS

• 25-Hydroxycholecalciferol is authorized as a feed additive in the European Union for use in poultry at maximum doses of 100 µg/kg in complete feed for fattening poultry and 80 µg/kg for other poultry [citation:2]
• It is also authorized for use in drinking water at maximum doses of 50 µg/L for fattening poultry and 40 µg/L for other poultry [citation:2]
• Since 2006, 25-OH-D3 has been permitted and widely used as another source of vitamin D in the European Union poultry industry [citation:4]

DOSAGE & ADMINISTRATION

SUPER HYDROXY D is administered via drinking water. For optimal results, fresh medicated water should be prepared daily.

Recommended Dosage:

• Poultry (Broilers, Layers, Breeders, Turkeys): 0.5 - 1 ml per 100-200 liters of drinking water (providing 3.7-7.4 µg 25-OH-D3 per liter)
• Alternative Calculation: Each ml treats 100-200 liters of drinking water
• Prevention: 0.5 ml per 200 liters of drinking water, administered 2-3 times per week
• Treatment of Deficiencies: 1 ml per 100 liters of drinking water for 5-7 consecutive days
• Stress Periods: 1 ml per 100-150 liters of drinking water for 3-5 days during vaccination, heat stress, or peak production
• Calculation: Each 1 liter container treats approximately 100,000-200,000 liters of drinking water at recommended concentrations

Recommended Usage Periods:

• Broiler Chickens: Administer during starter phase (0-14 days) to support skeletal development and growth; during finisher phase to support bone strength before processing
• Layers at Onset of Lay: Administer for 5-7 days as birds come into production to support eggshell quality
• Layers During Peak Production: Administer for 3-5 days monthly to maintain eggshell quality and bone health
• Layers in Late Production: Administer for 5-7 days to support calcium metabolism and eggshell quality as birds age
• Breeders: Administer for 5-7 days before and during the breeding season to improve fertility and hatchability
• Heat Stress: Administer during periods of high ambient temperature to maintain calcium balance
• Vaccination: Administer 1-2 days before and 2-3 days after vaccination to support immune response

Administration Guidelines:

• Calculate total daily water consumption based on flock size, age, and environmental temperature
• Dissolve the required amount of SUPER HYDROXY D directly into the drinking water tank or mixing system
• For use in automatic water proportioners, adjust settings according to manufacturer specifications
• Prepare fresh medicated water daily; discard any unused solution after 24 hours
• For best results, administer over 4-8 hours; may withhold water for 1-2 hours before treatment to encourage rapid consumption

CONTRAINDICATIONS & PRECAUTIONS

• Hypervitaminosis D: Do not exceed recommended dosage. Excessive vitamin D can cause hypercalcemia, soft tissue calcification, and toxicity. Adhere to maximum levels of 100 µg/kg in complete feed for fattening poultry [citation:2]
• Egg production: Safe for use in laying hens; no withdrawal period required for eggs when used at recommended levels
• Sensitive species: Use with caution in rabbits, hamsters, guinea pigs, or horses; consult a veterinarian before use
• Hypersensitivity: Do not use in animals with known hypersensitivity to vitamin D or any component
• Keep out of reach of children
• Store in a cool, dry place below 25°C, protected from direct sunlight
• Protect from freezing
• Shake well before use
• Use within 28 days after first opening

ADVERSE REACTIONS

• At recommended doses, the product is generally well tolerated in poultry
• Excessive intake may lead to hypervitaminosis D: hypercalcemia, reduced feed intake, soft tissue calcification, and potential kidney damage
• If adverse reactions occur, discontinue use and consult a veterinarian

WITHDRAWAL PERIOD

• Meat (Poultry): Zero days - no withdrawal period required when used according to label directions
• Eggs: Zero days - no withdrawal period required when used according to label directions

STORAGE

• Store in a cool, dry place below 25°C
• Protect from direct sunlight and freezing
• Keep container tightly closed when not in use
• Do not freeze

Target Species: Poultry (Broilers, Layers, Breeders, Turkeys, Ducks, Geese, Quail)

Pack Sizes: 1 Liter, 5 Liter HDPE containers

PRODUCT SUMMARY
SUPER HYDROXY D Liquid Nutraceutical 25-Hydroxycholecalciferol 7.4 µg per ml Poultry 1L,5L

FOR VETERINARY USE ONLY