Product Name: XCELSIO

Type: Bacteriophage Feed Additive

COMPOSITION

• Bacteriophage Cocktail – A blend of lytic bacteriophages specifically targeting:
• • Salmonella spp.
• • Escherichia coli
• • Staphylococcus aureus
• • Clostridium perfringens
• • Bacillus subtilis
• Carrier ...... q.s. to 1 kg

DESCRIPTION

XCELSIO is a cutting-edge bacteriophage-based feed additive developed through advanced research and development, utilizing a live cocktail of lytic bacteriophages to provide targeted control against specific pathogenic bacteria

What Are Bacteriophages? Bacteriophages (or "phages") are naturally occurring viruses that have evolved over millions of years to specifically target and eliminate only particular strains of bacteria, while leaving other beneficial bacteria completely unharmed

Mechanism of Action: Lytic bacteriophages—the type used in XCELSIO—attach to the surface of their target bacterial host, inject their genetic material, and hijack the bacterium's cellular machinery to replicate. The host bacterium is then lysed (destroyed), releasing hundreds of new phage particles that continue to seek out and destroy additional target bacteria. This self-perpetuating, self-limiting mechanism provides sustained pathogen reduction without disrupting the beneficial gut microbiota

Lytic vs. Temperate Phages: XCELSIO utilizes exclusively lytic bacteriophages, which have been genomically characterized to confirm the absence of lysogeny-related genes, antibiotic resistance genes, or virulence-associated genes—ensuring safety for the target animal, consumers, and the environment

Dual Serotype Activity: Research has identified lytic phages capable of dual serotype-specific activity against multiple Salmonella serovars, including S. Enteritidis and S. Typhimurium, with rapid adsorption rates and sustained inhibitory effects lasting up to 12 hours

Regulatory Milestone: In July 2025, the European Union officially approved the first-ever bacteriophage-based feed additive for poultry under Regulation (EC) No 1831/2003, following a rigorous safety and efficacy review by the European Food Safety Authority (EFSA) . The EFSA FEEDAP Panel concluded that such bacteriophage additives are safe for target species, consumers, and the environment, marking a significant regulatory breakthrough for phage technology in livestock production

EFFICACY DATA

Salmonella Control: A study evaluating bacteriophage cocktail supplementation in broilers infected with Salmonella gallinarum reported that a phage cocktail at concentration 10¹¹ PFU/ml was able to protect 100% of infected birds, with significantly reduced fecal shedding rates (1.2% vs. untreated group) and significant improvement in body weight gain

Another study demonstrated that dietary supplementation of bacteriophages at 1000 g/ton improved body weight gain and feed conversion ratio in both challenged and unchallenged chickens, while increasing beneficial Lactobacillus counts and decreasing pathogenic bacteria

Necrotic Enteritis (Clostridium perfringens) Control: A 2025 study evaluating bacteriophage supplementation in broilers challenged with Clostridium perfringens type G reported significant improvements: improved feed intake, body weight gain, and feed conversion ratio (p < 0.05); reduced intestinal lesion scores and mortality; restored jejunal histomorphology; and ameliorated blood biochemical disturbances

Summary of Efficacy Data (2024-2025):

INDICATIONS
XCELSIO is indicated for the prevention and control of bacterial infections caused by the following pathogens in livestock and poultry:

• Salmonella spp. – Including S. Enteritidis, S. Typhimurium, S. Gallinarum, and other serovars
• Escherichia coli – Including avian pathogenic E. coli (APEC) and other pathogenic strains
• Staphylococcus aureus – Including strains associated with mastitis and other infections
• Clostridium perfringens – Including NetB-positive strains causing necrotic enteritis in poultry
• Bacillus subtilis – Included for gut microbiota equilibrium support

Primary Applications:

• Antibiotic Alternative – A viable alternative to antibiotic growth promoters (AGPs) in antibiotic-free production systems
• Food Safety Enhancement – Reduction of foodborne pathogens (Salmonella, E. coli) at the pre-harvest stage
• Necrotic Enteritis Control – Management of C. perfringens infections in broiler production
• Gut Microbiota Equilibrium – Targeted pathogen reduction while preserving beneficial bacteria
• Performance Improvement – Enhanced feed efficiency, body weight gain, and reduced mortality

DOSAGE & ADMINISTRATION

For inclusion in feed or drinking water. Shake well before use if liquid formulation. Ensure uniform distribution when mixing into feed.

Recommended Inclusion Rates:

Administration Guidelines:

• Feed Application: Mix thoroughly into the complete feed using a progressive mixer to ensure uniform distribution
• Water Application: For drinking water administration, prepare fresh solution daily. Medicated water should be the only source of drinking water during treatment
• Prophylactic Use: Continuous supplementation during periods of high disease pressure or throughout the production cycle for optimal protection
• Therapeutic Use: Higher inclusion rates (1.0–2.0 kg/ton) may be used during active outbreaks under veterinary supervision
• Compatibility: Can be used in combination with probiotics; research shows synergistic effects when combined with probiotics for enhanced pathogen control

SAFETY & REGULATORY STATUS

• EFSA Approval (2025): The European Food Safety Authority (EFSA) has concluded that bacteriophage-based feed additives are safe for target species, consumers, and the environment, and has approved the first such product for use in all poultry species in the EU
• Genomic Safety: Bacteriophages in XCELSIO are genomically characterized to confirm the absence of lysogeny-related genes, antibiotic resistance genes, or virulence-associated genes
• No Residue Risk: Bacteriophages are self-limiting and degrade naturally in the environment; no withdrawal period is required
• Antibiotic Resistance: Does not contribute to antimicrobial resistance development; serves as a sustainable alternative to antibiotics
• Operator Safety: Bacteriophages are species-specific to bacteria and pose no risk to humans or animals. However, the additive should be considered a potential respiratory sensitiser; avoid inhalation of dust

WITHDRAWAL PERIODS

• Meat: 0 days (no withdrawal required)
• Milk: 0 hours (no withdrawal required)
• Eggs: 0 days (no withdrawal required)

PRECAUTIONS & STORAGE

• Store in a cool, dry place below 25°C, protected from direct sunlight and moisture
• Avoid exposure to high temperatures (>40°C) which may reduce phage viability
• Use within 6-12 months of opening for optimal phage titer
• Avoid mixing with highly acidic or alkaline substances that may affect phage stability
• Bacteriophages are heat-sensitive; avoid incorporation during feed pelleting at high temperatures (>80°C) unless encapsulated
• May be considered a potential respiratory sensitiser; use appropriate dust control measures during handling
• No known contraindications when used as directed
• Keep out of reach of children.

Target Species: Poultry (Broilers, Layers, Turkeys), Swine, Cattle (Calves)

Pack Sizes: 1 kg, 20 kg

PRODUCT SUMMARY
XCELSIO Bacteriophage Feed Additive Powder Bacteriophages targeting: Salmonella spp., E. coli, Staphylococcus aureus, Clostridium perfringens, Bacillus subtilis Livestock 1 Kg,20Kg

FOR VETERINARY USE ONLY