Product Name: XCELSIO wsp

Type: Bacteriophage (Water Soluble Powder)

COMPOSITION

• Bacteriophage Cocktail – A blend of lytic bacteriophages specifically targeting:
• • Salmonella spp. (including S. Enteritidis and S. Typhimurium)
• • Escherichia coli (including avian pathogenic strains)
• • Staphylococcus aureus
• • Clostridium perfringens (including NetB-positive strains)
• • Bacillus subtilis
• Water-Soluble Carrier ...... q.s. to 1 kg

DESCRIPTION

XCELSIO wsp is a water-soluble bacteriophage-based feed additive formulated for convenient administration via drinking water. It contains a live cocktail of lytic bacteriophages that specifically target and eliminate key pathogenic bacteria while preserving beneficial gut microbiota

What Are Bacteriophages? Bacteriophages (or "phages") are viruses that infect and replicate only in bacterial cells. They are ubiquitous in the environment and recognized as the earth's most abundant biological agent, outnumbering bacteria by approximately 10:1

Mechanism of Action: Lytic Replication XCELSIO wsp utilizes exclusively lytic (virulent) bacteriophages, which follow a distinct lifecycle

Adsorption: The phage attaches to specific receptors on the surface of its target bacterial host using specialized tail fibers. This specificity ensures that only pathogenic bacteria are targeted

Injection: The phage injects its genetic material (DNA or RNA) into the bacterial cell cytoplasm

Replication & Assembly: The phage hijacks the host bacterium's cellular machinery to synthesize new phage components. Multiple copies of the phage are assembled within the bacterial cell

Lysis: The host bacterium is lysed (destroyed), releasing hundreds of new phage particles that continue to seek out and destroy additional target bacteria. This self-perpetuating mechanism provides sustained pathogen reduction

This lytic replication cycle is self-limiting—when the target bacterial population is reduced, phage numbers naturally decline as they lose their hosts

Lytic vs. Temperate Phages: XCELSIO wsp utilizes exclusively lytic bacteriophages, which kill their host bacteria upon replication. This is distinct from temperate phages, which can integrate into the bacterial genome (lysogenic cycle) without immediately killing the host

Water-Soluble Powder Formulation: As a water-soluble powder (also known as a soluble powder or "wsp"), this product is designed to dissolve completely in water, allowing for easy and uniform administration via drinking water systems. This formulation is particularly advantageous for poultry and swine operations where drinking water is the primary administration route

Regulatory Milestone: In July 2025, the European Union officially approved the first-ever bacteriophage-based feed additive for poultry under Regulation (EC) No 1831/2003. The EFSA FEEDAP Panel concluded that such bacteriophage additives are safe for target species, consumers, and the environment

EFFICACY DATA

Salmonella Control: A study evaluating bacteriophage cocktail supplementation in broilers infected with Salmonella gallinarum reported that a phage cocktail at concentration 10¹¹ PFU/ml was able to protect 100% of infected birds, with significantly reduced fecal shedding rates (1.2% vs. untreated group) and significant improvement in body weight gain

Another study demonstrated that dietary supplementation of bacteriophages at 1000 g/ton improved body weight gain and feed conversion ratio in both challenged and unchallenged chickens, while increasing beneficial Lactobacillus counts and decreasing pathogenic bacteria

Necrotic Enteritis (Clostridium perfringens) Control: A 2025 study evaluating bacteriophage supplementation in broilers challenged with Clostridium perfringens type G reported: improved feed intake, body weight gain, and feed conversion ratio (p < 0.05); reduced intestinal lesion scores and mortality; restored jejunal histomorphology; and ameliorated blood biochemical disturbances

Summary of Efficacy Data:

INDICATIONS
XCELSIO wsp is indicated for the prevention and control of bacterial infections caused by the following pathogens in livestock and poultry:

• Salmonella spp. – Including S. Enteritidis, S. Typhimurium, S. Gallinarum, and other serovars
• Escherichia coli – Including avian pathogenic E. coli (APEC) and other pathogenic strains
• Staphylococcus aureus – Including strains associated with mastitis and other infections
• Clostridium perfringens – Including NetB-positive strains causing necrotic enteritis in poultry
• Bacillus subtilis – Included for gut microbiota equilibrium support

Primary Applications:

• Antibiotic Alternative – A viable alternative to antibiotic growth promoters (AGPs) in antibiotic-free production systems
• Food Safety Enhancement – Reduction of foodborne pathogens (Salmonella, E. coli) at the pre-harvest stage
• Necrotic Enteritis Control – Management of C. perfringens infections in broiler production
• Gut Microbiota Equilibrium – Targeted pathogen reduction while preserving beneficial bacteria
• Performance Improvement – Enhanced feed efficiency, body weight gain, and reduced mortality

DOSAGE & ADMINISTRATION

For administration via drinking water. Prepare fresh solution daily. The water-soluble powder dissolves completely in water for uniform distribution.

Recommended Inclusion Rates:

Administration Guidelines:

• Prepare Fresh Daily: Dissolve the required amount in clean drinking water. Medicated water should be the only source of drinking water during treatment
• Duration: Administer continuously for 3-7 days or as directed by a veterinarian. For preventive use, can be administered 2-3 days per week
• Water System Compatibility: Suitable for use with all standard drinking water systems including nipple drinkers, bell drinkers, and troughs
• Prophylactic Use: Continuous or intermittent supplementation during periods of high disease pressure or throughout the production cycle for optimal protection
• Therapeutic Use: Higher inclusion rates (1.0–2.0 g per liter) may be used during active outbreaks under veterinary supervision
• Compatibility: Can be used in combination with probiotics; research shows synergistic effects when combined with probiotics for enhanced pathogen control

ADVANTAGES OF WATER SOLUBLE FORMULATION

• Easy Administration: Simple mixing into drinking water eliminates need for feed processing
• Uniform Distribution: Ensures all animals receive the correct dose regardless of feed intake
• Rapid Action: Direct delivery to the gastrointestinal tract via drinking water
• Heat Stability: Avoids potential heat degradation that may occur during feed pelleting
• Sick Animal Treatment: Ideal for administering to animals with reduced feed intake but continued water consumption

SAFETY & REGULATORY STATUS

• EFSA Approval (2025): The European Food Safety Authority (EFSA) has concluded that bacteriophage-based feed additives are safe for target species, consumers, and the environment
• Genomic Safety: Bacteriophages in XCELSIO wsp are genomically characterized to confirm the absence of lysogeny-related genes, antibiotic resistance genes, or virulence-associated genes
• No Residue Risk: Bacteriophages are self-limiting and degrade naturally in the environment; no withdrawal period is required
• Antibiotic Resistance: Does not contribute to antimicrobial resistance development; serves as a sustainable alternative to antibiotics
• Operator Safety: Bacteriophages are species-specific to bacteria and pose no risk to humans or animals. However, the additive should be considered a potential respiratory sensitiser; avoid inhalation of dust

WITHDRAWAL PERIODS

• Meat: 0 days (no withdrawal required)
• Milk: 0 hours (no withdrawal required)
• Eggs: 0 days (no withdrawal required)

PRECAUTIONS & STORAGE

• Store in a cool, dry place below 25°C, protected from direct sunlight and moisture
• Avoid exposure to high temperatures (>40°C) which may reduce phage viability
• Use within 6-12 months of opening for optimal phage titer
• Avoid mixing with highly acidic or alkaline water sources (pH 5.0-8.0 is optimal for phage stability)
• If using chlorinated water, allow chlorine to dissipate or treat with a dechlorinator before adding bacteriophages, as chlorine may reduce phage viability
• Prepare fresh medicated water daily; discard any remaining medicated water after 24 hours
• May be considered a potential respiratory sensitiser; use appropriate dust control measures during handling
• No known contraindications when used as directed
• Keep out of reach of children.

Target Species: Poultry (Broilers, Layers, Turkeys), Swine, Cattle (Calves)

Pack Sizes: 1 kg, 20 kg

PRODUCT SUMMARY
XCELSIO wsp Bacteriophage Water Soluble Powder Bacteriophages targeting: Salmonella spp., E. coli, Staphylococcus aureus, Clostridium perfringens, Bacillus subtilis Livestock 1 Kg,20Kg

FOR VETERINARY USE ONLY